The Colifast At-Line Monitor – CALM is a fully automated early warning system for monitoring of coliforms and Pseudomonas Aeruginosa in water. Detection time ranges from 2 to 12 hours. The technology is timesaving, cost reducing, and environmentally friendly. Examples of CALM applications are monitoring of raw water, in-process water, waste water and recreational water. No laboratory facilities and minimal skills in microbiology are required to perform the analysis. The CALM is easily operated by the system computer or via LAN interface. The patented Colifast growth media are supplied in pre-filled multi well Trays™ and the flexibility if the system makes it easy to adapt to different applications. The CALM can perform parallel analysis of different target organisms. Depending on method and test frequency, the CALM needs monthly or weekly refilling of reagents.
The CALM detects and quantifies E. coli, thermotolerant coliforms and total coliforms in water by fluorometrical monitoring of B-glucoronidase activity. The detection of P. aeruginosa is based on substrate hydrolysation by amino peptidase enzymes present in this bacterium. The Colifast technology is comprised of unique media formulations combined with instrument detection systems. The technology is covered by patents in numerous countries, with several additional international patents pending. Incubator temperatures for the selection of thermotolerant coliforms (44 °C) and total coliforms / E. coli (37 °C) / P. aeruginosa (37 °C) is present.